Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells.
Identifieur interne : 000148 ( Main/Exploration ); précédent : 000147; suivant : 000149Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells.
Auteurs : Kiran Suhas Nilangekar [Inde] ; Bhupendra V. Shravage [Inde]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2019.
Descripteurs français
- KwdFr :
- Animal génétiquement modifié (génétique), Animal génétiquement modifié (métabolisme), Animaux (MeSH), Cellules souches germinales femelles (métabolisme), Communication cellulaire (MeSH), Drosophila melanogaster (génétique), Drosophila melanogaster (métabolisme), Espèces réactives de l'oxygène (analyse), Femelle (MeSH), Glutarédoxines (génétique), Glutarédoxines (métabolisme), Gènes rapporteurs (MeSH), Mitochondries (génétique), Mitochondries (métabolisme), Oxydoréduction (MeSH), Protéines de Drosophila (génétique), Protéines de fusion recombinantes (métabolisme), Protéines de liaison à l'ARN (génétique), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Régions promotrices (génétique) (MeSH), Techniques de biocapteur (MeSH).
- MESH :
- analyse : Espèces réactives de l'oxygène.
- génétique : Animal génétiquement modifié, Drosophila melanogaster, Glutarédoxines, Mitochondries, Protéines de Drosophila, Protéines de liaison à l'ARN, Protéines à fluorescence verte.
- métabolisme : Animal génétiquement modifié, Cellules souches germinales femelles, Drosophila melanogaster, Glutarédoxines, Mitochondries, Protéines de fusion recombinantes, Protéines à fluorescence verte.
- Animaux, Communication cellulaire, Femelle, Gènes rapporteurs, Oxydoréduction, Régions promotrices (génétique), Techniques de biocapteur.
English descriptors
- KwdEn :
- Animals (MeSH), Animals, Genetically Modified (genetics), Animals, Genetically Modified (metabolism), Biosensing Techniques (MeSH), Cell Communication (MeSH), Drosophila Proteins (genetics), Drosophila melanogaster (genetics), Drosophila melanogaster (metabolism), Female (MeSH), Genes, Reporter (MeSH), Glutaredoxins (genetics), Glutaredoxins (metabolism), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Mitochondria (genetics), Mitochondria (metabolism), Mitophagy (MeSH), Oogonial Stem Cells (metabolism), Oxidation-Reduction (MeSH), Promoter Regions, Genetic (MeSH), RNA-Binding Proteins (genetics), Reactive Oxygen Species (analysis), Recombinant Fusion Proteins (metabolism).
- MESH :
- chemical , analysis : Reactive Oxygen Species.
- chemical , genetics : Drosophila Proteins, Glutaredoxins, Green Fluorescent Proteins, RNA-Binding Proteins.
- genetics : Animals, Genetically Modified, Drosophila melanogaster, Mitochondria.
- metabolism : Animals, Genetically Modified, Drosophila melanogaster, Glutaredoxins, Green Fluorescent Proteins, Mitochondria, Oogonial Stem Cells, Recombinant Fusion Proteins.
- Animals, Biosensing Techniques, Cell Communication, Female, Genes, Reporter, Mitophagy, Oxidation-Reduction, Promoter Regions, Genetic.
Abstract
Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. Monitoring mROS levels is therefore essential to understand cell-cell communication and programmed cell death in all types of cell including stem cells. Here, we describe generation and characterization of a redox sensor for mROS that is specifically expressed in the germline stem cells (GSCs) in Drosophila. This redox sensor can be used to monitor the production of mROS and mitophagy in the GSCs during oogenesis.
DOI: 10.1007/7651_2018_167
PubMed: 30027507
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(génétique)</term><term>Drosophila melanogaster (métabolisme)</term><term>Espèces réactives de l'oxygène (analyse)</term><term>Femelle (MeSH)</term><term>Glutarédoxines (génétique)</term><term>Glutarédoxines (métabolisme)</term><term>Gènes rapporteurs (MeSH)</term><term>Mitochondries (génétique)</term><term>Mitochondries (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Protéines de Drosophila (génétique)</term><term>Protéines de fusion recombinantes (métabolisme)</term><term>Protéines de liaison à l'ARN (génétique)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Techniques de biocapteur (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Drosophila 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type="abstract" xml:lang="en">Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. Monitoring mROS levels is therefore essential to understand cell-cell communication and programmed cell death in all types of cell including stem cells. Here, we describe generation and characterization of a redox sensor for mROS that is specifically expressed in the germline stem cells (GSCs) in Drosophila. This redox sensor can be used to monitor the production of mROS and mitophagy in the GSCs during oogenesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30027507</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1854</Volume><PubDate><Year>2019</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells.</ArticleTitle><Pagination><MedlinePgn>13-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/7651_2018_167</ELocationID><Abstract><AbstractText>Mitochondrial reactive oxygen species (mROS), a major source of ROS within cells, functions as an important signaling molecule and has the ability to damage cellular macromolecules including DNA and proteins. Monitoring mROS levels is therefore essential to understand cell-cell communication and programmed cell death in all types of cell including stem cells. Here, we describe generation and characterization of a redox sensor for mROS that is specifically expressed in the germline stem cells (GSCs) in Drosophila. This redox sensor can be used to monitor the production of mROS and mitophagy in the GSCs during oogenesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nilangekar</LastName><ForeName>Kiran Suhas</ForeName><Initials>KS</Initials><AffiliationInfo><Affiliation>Developmental Biology Group, MACS-Agharkar Research Institute, Pune, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shravage</LastName><ForeName>Bhupendra V</ForeName><Initials>BV</Initials><AffiliationInfo><Affiliation>Developmental Biology Group, MACS-Agharkar Research Institute, Pune, India. bvshravage@aripune.org.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol 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